Lipofectamine 3000 p3000 reagent. Apr 30, 2024 · Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. Without P3000 reagent in the Shared reagents DNA transfection siRNA transfection Growth medium Opti-MEM medium for complexing DNA P3000 reagent Lipofectamine 3000 reagent** siRNA Lipofectamine 3000 reagent** 96-well 0. 37, 0. 5 μL: 2: Tube 2: Opti-MEM I medium: 25 μL: DNA amount (DNA concentration should be 0. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. 75 mL) and one vial of P3000™ Enhancer Reagent. 6 $ Add DNA-lipid comple x to cells Component (per well) 96-well 24-well DNA-lipid complex 10 ˚L50 DNA amount 100 ng 500 ng P3000™ Reagent 0. 75 μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells. 5 μL: P3000™ reagent: 1 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Lipofectamine 3000 reagent is designed to provide: Superior efficiency—for the broadest spectrum of difficult-to-transfect cells. Specifically for HEK293 cells, transfect cells with 1 μg Myc-MG53 plasmid using 2 μL P3000™ Reagent and 3 μL Lipofectamine™ 3000 Reagent per 3. 25 μL: 2: Tube 2: Opti-MEM I medium: 25 μL: DNA amount (DNA concentration should be 0. Use the indicated volume of DNA and P3000™ Reagent with each P3000™ Reagent (2 μL/μg DNA) 0. Here, we demonstrate the effectiveness of Lipofectamine 3000 reagent for the production of high-quality, high-titer lentivirus while minimizing time and reagent usage. 75 µL Lipofectamine 3000 reagent 1. 75 µL Lipofectamine 3000 reagent is designed to provide: Superior efficiency—for the broadest spectrum of difficult-to-transfect cells. 3 3 0. 15 µL 3 pmol 0. Enable High Transfection Efficiency in Novel Genome Editing Lipofectamine 3000 transfection reagent also improves the cleavage efficiency with CRISPR vectors on pluripotent stem cells, yielding a 2-fold increase in the efficiency of genetic modifications. 5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; Lipofectamine 3000 reagent 0. Eine Lipiddosis mit niedriger Toxizität (0,75 μl für 24-Well-Platten) wird für Anwendungen empfohlen, bei denen eine minimale Zellunterbrechung erforderlich ist. Lipofectamine 3000 reagent: 1 μL: 2: Tube 2: Opti-MEM I medium: 25 μL: DNA amount (DNA concentration should be 0. Enable High Transfection Efficiency in Novel Genome Editing Lipofectamine ™ Reagents Go online to view related products. 5–5 µg/µL) 250 ng P3000™reagent 0. Store at 2–8°C. 5 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0. 5–5 μg/μL) 500 ng: P3000™ reagent: 1 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Thermo Fisher Scientific. 6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks. Lipofectamine 3000 reagent 0. Transfecting Plasmid DNA Into JC Cells Using Lipofectamine 3000 Reagent | Thermo Fisher Scientific - MH Shared reagents DNA transfection siRNA transfection Growth medium Opti-MEM medium for complexing DNA P3000 reagent Lipofectamine 3000 reagent** siRNA Lipofectamine 3000 reagent** 96-well 0. 75μL for 24-well plates) is suggested for applications requiring minimal disruption of the cells. Make DNA-Lipofectamine ™ 3000 complexes in serum-free medium such Steps. 5 µL 0. The first reagent is placed in a tube with OPTIMEM media. Contains one vial of Lipofectamine® 3000 Reagent (0. For common cell types, Lipofectamine 3000 reagent is more cost-effective than other reagents due to its high concentration and low usage amount. The easy protocol, exceptional plasmid DNA delivery, and the ability to Shared reagents DNA transfection siRNA transfection Growth medium Opti-MEM medium for complexing DNA P3000 reagent Lipofectamine 3000 reagent** siRNA Lipofectamine 3000 reagent** 96-well 0. Here, we demonstrate the effectiveness of Lipofectamine 3000 reagent for the production of high-quality Diluted Lipofectamine ® 3000 Reagent 5 ˚L 25 ˚L 5 $ Incubate Incubate for 5 minutes at room temperatu e. 2 100 μL 2 x 5 μL 100 ng 0. Versatility—single kit for DNA, RNA, and cotransfection. This got me wondering, what is the P3000 reagent? What additional perks, if you will, does it Lipofectamine 3000 comprises of two reagents: Lipofectamine 3000 and P3000. 5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; Shared Reagents DNA Transfection siRNA Transfection Vol. 3 µL 3 pmol 0. The 1. As a result, less optimization is necessary. 25 µL 0. Prepare DNA master mix by diluting DNA in Opti-MEM medium, then add P3000 reagent—mix well. 2 µL 3 pmol 0. Therefore, our results suggest that a much higher transfection and viral transduction efficiency can be attained by using Lipofectamine 3000 transfection reagent. ZERO BIAS - scores, article reviews, protocol conditions and more Aug 15, 2015 · a) Tube 1: Dilute 100ng DNA ( high quality, endotoxin-free) + 0. Contents of both tubes are combined and mixed prior to incubation for 15 minutes. The Optimem is sensitive to Lipofectamine 3000 reagent 2 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0. g. 75 µL Lipofectamine 3000 reagent: 1. Transfect cells according to the following table. 75 µL Lipofectamine ™ Reagents Go online to view related products. 5–5 µg/µL) 500 ng P3000™ reagent 1 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; . A second tube contains P3000 with OPTIMEM, and DNA of choice. To prepare lipid-DNA complexes, transfer contents of Tube A to Tube B and mix well. (B) Summary of fold increases in titer levels using two different cell lines and insert Lipofectamine™ 3000試薬は、遺伝子導入が困難な細胞にも効率的にトランスフェクションできるように設計されており、幅広い細胞種にわたって卓越したトランスフェクション性能が得られます。次のような細胞種に良好にトランスフェクションできます。 Lipofectamine ™ Reagents Go online to view related products. 5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; Lipofectamine 3000 reagent: 1 μL: 2: Tube 2: Opti-MEM I medium: 25 μL: DNA amount (DNA concentration should be 0. 5mL size provides up to 1500 transfection reactions (in 24-well plates). Browse these related products instead Lipofectamine 3000 reagent: 1 μL: 2: Tube 2: Opti-MEM I medium: 25 μL: DNA amount (DNA concentration should be 0. 1 µg 0. 2µL 1µL 5µL Lipofectamine 3000 reagent per well 0. Each reagent was used to transfect HEK 293, HeLa, LNCaP, A549, and HepG2 cells in a 96-well format, and GFP expression was analyzed 48 hours posttransfection. Rating: 4. To tube A add 6µl of lipofectamine 3000 (1µg DNA : 3µl ) vortex briefly and spin down. Enable High Transfection Efficiency in Novel Genome Editing This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e. Enable High Transfection Efficiency in Novel Genome Editing Apr 20, 2018 · Using Cx43 shRNA lentivirus combined with Lipofectamine 3000 transfection reagent, we can achieve about 90% Cx43 knockdown efficacy in HUVECs. Then 100 µL P3000 Reagent per well 0. Bioz Stars score: 86/100, based on 1 PubMed citations. 5–5 μg/μL) 250 ng: P3000™ reagent: 0. 5 Day 2 – 4 7 Lipofectamine 3000 reagent can deliver DNA into difficult-to-transfect differentiated cells in culture. 5µL 96 well 24 well 6 well E 1 2 3 Transfection amounts Protocol Transfect cells according to the following table. ∤. Growth Medium Vol. Opti-MEM for complexing DNA (μg) P3000™ (μL) Lipofectamine® 3000 Reagent2 (μL) siRNA (pmol) Lipofectamine® 3000 Reagent2 (μL) 96-well 0. Do not freeze. (B) Summary of fold increases in titer levels using two different cell lines and insert Lipofectamine 3000 reagent: 1. (A) Titers obtained using Lipofectamine 3000 reagent compared to commonly used reagents in 293FT cells using a 3kb insert gene. The reagent allows for transfection to be performed both in presence or absence of serum. Lipofectamine 3000 reagent for pluripotent stem cell applications. 75 µL Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. Enable High Transfection Efficiency in Novel Genome Editing Poster: Lipofectamine 3000 reagent efficient, reproducible Shared Reagents DNA Transfection siRNA Transfection Vol. Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. Lipofectamine 3000 reagent: 1. 3ul Lipofectamine 3000 reagent in 5ul Opti-MEM I. Application Area: Over-expression of protein. 25 0. 75 7. A low toxicity lipid dose (0. 5 pmol 0. Lipomaster 3000 Reagent结构明确，可实现高效、高重复性的细胞转染, T3000 Enhancer Reagent作为转染试剂增强剂可以辅助DNA快速入核，两者搭配使用对大片段质粒 Lipofectamine 3000 reagent 0. 5 mL) for 1000-1500 reactions in 24-well plates, or 200-250 reactions in 6-well plates. + $ Add DNAl ipid complex to cells Das Lipofectamine™ 3000-Reagenz sorgt für eine hohe Transfektionseffizienz in einem robusten dynamischen Bereich von Lipiddosen für eine schnelle und einfache Optimierung. 5 μL: P3000™ reagent: 1 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Lipofectamine 3000 reagent 1. The next day, transfect cells with plasmids using Lipofectamine™ 3000 Reagent following manufacturer’s instructions. 5 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Which one is more toxic between Lipofectamine 3000 and p3000. 5 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Lipofectamine 3000 Reagent User Guide, supplied by Thermo Fisher, used in various techniques. Lipofectamine 3000 reagent: 0. 5–5 μg/μL) 0. 25 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0. 4 μL 2 μL 10 μL) $ Add Diluted DNA to each tube of Diluted LipofectamineÆ 3000 Reagent (1:1 ratio) Diluted DNA (with P3000™ Reagent) 5 μL25 125 Diluted Lipofectamine® 3000 Reagent 5 μL 25 μL 125 μL * $ Incubate Incubate for 5 minutes at room temperature. See full list on thermofisher. This mixture is added to cells to allow for tranfection to occur. Longer times can result in a loss of transfection efficiency. To tube B add 4µl of P3000 reagent (2µl : µg DNA Lipofectamine 3000 reagent and 1 µg of DNA were each diluted in 50 µL Gibco™ Opti-MEM™ Reduced-Serum Medium; then 2 µL P3000™ reagent was added to the diluted DNA. 2ul of P3000 reagent in 5ul Opti-MEM I. The supplier does not provide quotations for this product through SelectScience. 5 cm dish according to manufacturer’s instructions. 5 µL 7. The easy protocol, exceptional plasmid DNA delivery, and the ability to Lipofectamine 3000 reagent: 1. 이 시약을 사용하면 가장 폭넓으면서도 transfection이 어려운 일반 세포 (예: HEK293, HeLa)에서 뛰어난 transfection When you perform DNA transfection using Lipofectamine 3000, diluted DNA need mix with P3000 before combining with diluted Lipofectamine 3000 reagent to make complex. The diluted DNA with P3000 reagent was added to the diluted Lipofectamine 3000 reagent and incubated at room temperature for 5 minutes. 5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to Pancreatic carcinoma (PANC-1) Skin melanoma (SK-MEL-28) Transfect Common Cells Efficiently and Cost-Effectively. Make DNA-Lipofectamine ™ 3000 complexes in serum-free medium such But in other thermo fisher protocols, it says to use the P3000 reagent with the lipofectamine 3000. Browse these related products instead Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. Incubate the complexes for 10–20 minutes at room temperature. 17 100 μL 2 × 5 μL 0. Lipofectamine™ 3000 Transfection Reagent 는 최첨단 지질 나노입자 기술을 활용해 이입 성능을 강화하고 어플리케이션 결과와 재현성을 향상시켜 줍니다. 50 250 μL 2 × 12. Add diluted DNA to each tube of diluted Lipofectamine 3000 reagent (1:1 ratio) Incubate. Advantages of lentiviral production using Lipofectamine 3000 reagent: Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. 75 Lipofectamine 3000 reagent performs significantly better than leading competitor transfection reagents for lentiviral production. Use the indicated volume of DNA and P3000™ Reagent with each of the two volumes of Lipofectamine™ 3000 (when performing optimization). Format. 5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; 1 Tube 1 Opti-MEM™I medium 25 µL Lipofectamine 3000 reagent 1 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0. トランスフェクションが困難な細胞や一般的な細胞など幅広い細胞タイプで、非常に優れたトランスフェクション効率を Also, I used 2500 ng of DNA per well, 5µl of P3000 per DNA, 5µl of Lipofectamine 3000, and DMEM with P/s + L-glu instead of OMEM. 75 µL Lipomaster 3000 Transfection Reagent是一种由脂质体介导的转染试剂，适用于多种贴壁或悬浮细胞的质粒DNA、RNA转染。. In my modicum of knowledge, p3000 acts as an enhancer . 25 µg 0. Note: Lipofectamine 3000 reagent diluted in Opti-MEM medium should be used within 15 minutes of dilution. 38 µL 7. * Designed by Dr. FAQ. However, after 48 hours of transfections majority of the cells Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable exceptional transfection performance and reproducible results. 5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; Lipofectamine 3000 reagent and 1 µg of DNA were each diluted in 50 µL Gibco™ Opti-MEM™ Reduced-Serum Medium; then 2 µL P3000™ reagent was added to the diluted DNA. “Successfully transfected GT1-1 cells. Lipofectamine ™ 3000 Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells and especially designed for hard to transfect cells. Make two 125µl aliquots of opti-MEM ( A and B) 5. c) Combine the two tubes and incubate at room temperature for 5 minutes to form transfection complex. 1 mL) and one vial of P3000™ Enhancer Reagent. 5–5 µg/µL) 500 ng P3000™ reagent 1 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; Lipofectamine 3000 reagent is a specially formulated gentle transfection reagent that is optimized for plasmid delivery into CHO cells with high efficiency and low cell toxicity. 5 0. 5 mL) and one vial of P3000™ Enhancer Reagent. It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 图5. 5–5 µg/µL) 250 ng P3000™ reagent 0. But in other thermo fisher protocols, it says to use the P3000 reagent Contains one vial of Lipofectamine® 3000 Reagent (0. 1 0. 5–5 μg/μL) 500 ng: P3000™ reagent: 1 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Lipofectamine 3000 reagent leverages our most advanced lipid nanoparticle technology to enable exceptional transfection performance and reproducible results. 2 100 μL 2 x 5 μL 50 ng 0. 3 48-well 0. 5 μL 0. , HEK293, HeLa). (2) Prepare tube ‘A’ and tube ‘B’ (following the Lipofectamine 3000 protocol); (3) Wait for 5 minutes May 5, 2019 · Lipofectamine 3000 reagent per well 0. 2 100 μL 2 x 5 μL 0. Lipofectamine ® Lipofectamine 3000 reagent 1 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0. Important Guidelines. Low toxicity—gentle on cells for improved viability. 2 0. Transfection Technique. 75 μL: 2: Tube 2: Opti-MEM I medium: 25 μL: DNA amount (DNA concentration should be 0. 3. 5 250 μL 2 x 12. We would like to show you a description here but the site won’t allow us. 5 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. 5 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; Optimized protocol and tips & tricks for transfecting JC breast cancer cells in a 24-well plate using Lipofectamine 3000 reagent. A workflow is provided in Figure 2, followed by a more detailed protocol. This versatile reagent enables high viral titers even with genes that are large or difficult to package. 15, 0. Sep 17, 2021 · b. Lipofectamine 3000试剂盒电转染的重编程效率比较。使用Lipofectamine 3000 试剂或Neon转染系统将Epi5™载体转染进入BJ成纤维细胞以及新生儿（HDFn）和成人（HDFa）皮肤成纤维细胞并将其重编程为iPSC。采用明场显微镜和碱性磷酸酶(B) 染色法，对克隆进行(A)可视化。 Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. 5µL 3. Seed cells to be 70–90% confluent at transfection. Lipofectamine 3000 reagent can deliver DNA into difficult-to-transfect Lipofectamine 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. b) Tube 2: Dilute 0. The easy protocol, exceptional plasmid DNA delivery, and the ability to According to ThermoFisher, lipofectamine 3000 can be used until 2 years after the manufacturing date if stored properly (stored in a 4 degree fridge should be fine). 2 µL 0. 75 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0. 2 ˚L 1 ˚L Lipofectamine ® 3000 Reagent used 0. so try using less reagent, and optionally incorporate media Lipofectamine 3000 reagent 1. 3 µL 48-well 0. Lipofectamine 3000 reagent can deliver DNA into ESCs and iPSCs in culture. Never achieved with any other reagent except viruses and Lipofectamine 3000試薬は、最先端の脂質ナノ粒子技術を活用して、優れたトランスフェクション効率と再現性の高い結果をもたらします。. 5–5 µg/µL) 500 ng P3000™ reagent 1 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; Lipofectamine 3000 reagent 0. 6. Never achieved with any other reagent except viruses and Shared reagents DNA transfection siRNA transfection Growth medium Opti-MEM medium for complexing DNA P3000 reagent Lipofectamine 3000 reagent** siRNA Lipofectamine 3000 reagent** 96-well 0. Figure 1. Add DNA– lipid complex to Lipofectamine 3000 reagent: 2 μL: 2: Tube 2: Opti-MEM I medium: 25 μL: DNA amount (DNA concentration should be 0. 15 and 0. 375 µL 7. Achieve Superior Transfection Efficiency While Maintaining Low Toxicity Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. 3 ˚L 0. Make DNA-Lipofectamine ™ 3000 complexes in serum-free medium such Lipofectamine 3000 reagent performs significantly better than leading competitor transfection reagents for lentiviral production. For vector DNA–based RNAi applications, we recommend Lipofectamine™ 3000 Reagent with the P3000™ Enhancer Reagent. Dilute Lipofectamine 3000 reagent in Opti-MEM medium. 75 µL Achieve Superior Transfection Efficiency While Maintaining Low Toxicity Lipofectamine™ 3000 reagent maintains a high transfection efficiency within a robust dynamic range of lipid doses for quick and easy optimization. 75 µL 7. 5 mL) and one vial of P3000TM Enhancer Reagent (1. 75 and 7. com It has been specifically developed for siRNA transfection while providing high transfection efficiency with minimal cytotoxicity. 3µL 0. Lipofectamine 3000 provides excellent plasmid over-expression, sans cell toxicity. (2 tubes)—mix well. " Lipofectamine 3000 provided gentle but efficient transfection in several line of interest to our lab, HEI-OC1 mouse cochlear (69-fold) , NCCIT and Ntera2 (3 fold) testicular cancer lines and allowed for 本文详细介绍了lipo3000转染试剂的使用方法和注意事项，帮助实验者提高转染效率和成功率，同时对比了其他常用的转染试剂 Lipofectamine 3000 reagent 1 µL 2 Tube 2 Opti-MEM I medium 25 µL DNA amount (DNA concentration should be 0. 75 Invitrogen™ Lipofectamine™ 3000 Transfection Reagent is a highly efficient, cost-effective tool for lentiviral production (Figure 1). 125 µg 0. Then 100 µL 3000 was developed to be easy to use while still ensuring optimum performance and reliability in a wide panel of cell lines. 5 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Shared reagents DNA transfection siRNA transfection Growth medium Opti-MEM medium for complexing DNA P3000 reagent Lipofectamine 3000 reagent** siRNA Lipofectamine 3000 reagent** 96-well 0. Contains one vial of Lipofectamine™ 3000 Reagent (1. 75 and 1. 1 µL 0. 5–5 µg/µL) 500 ng P3000™ reagent 1 µL 3 Add tube 2 solution to tube 1 and mix well 4 Incubate mixture from step 3 at room temperature for 10–15 min 5 Add 50 µL of complex from step 4 to cells; We would like to show you a description here but the site won’t allow us. 5µL 96 well 24 well 6 well 1 2 3 Transfection Amounts Protocol 7. It delivers exceptional transfection efficiency with improved cell viability for the widest range of hard-to-transfect and common cell types including HEK 293, HeLa, LNCaP, HepG2, and A549 Oct 9, 2023 · reagent, p3000, considering all the wells for the transfection experiment. 5 μL: P3000™ reagent: 1 μL: 3: Add tube 2 solution to tube 1 and mix well: 4: Incubate mixture from step 3 at room temperature for 10–15 min: 5: Add 50 μL of complex from step 4 to cells; Contains one vial of Lipofectamine® 3000 Reagent (1. Browse these related products instead Shared reagents DNA transfection siRNA transfection Growth medium Opti-MEM medium for complexing DNA P3000 reagent Lipofectamine 3000 reagent** siRNA Lipofectamine 3000 reagent** 96-well 0. HeLa are easy to transfect and typically require less Lipofectamine 2000 or 3000 - vs other "tougher" cells. Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. hc bh gl le yr px yp ur td pv